Home > Sandwich ELISA Kits > Human Hepatocyte Growth Factor Receptor (HGFR) ELISA Kit

Product Name:

Human Hepatocyte Growth Factor Receptor (HGFR) ELISA Kit

Cat.#:

JL19411

Regulatory Status:

RUO

Alternative:

C-Met; RCCP2; Hepatocyte Growth Factor Receptor; Met Proto-Oncogene; Mesenchymal-Epithelial Transition Factor; HGF/SF receptor; Proto-oncogene c-Met; Scatter factor receptor; Tyrosine-protein kinase M

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.12ng/mL

Specificity:

This kit recognizes Human HGFRin samples. No significant cross-reactivity or interference between Human HGFR and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Assay Procedure:

Research Area:

Cancer

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| HGFR

Hepatocyte growth factor receptor (HGF receptor) is a protein that in humans is encoded by the MET gene. The protein possesses tyrosine kinase activity.The primary single chain precursor protein is post-translationally cleaved to produce the alpha and beta subunits, which are disulfide linked to form the mature receptor.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.62	0.31	0
OD	2.44	1.91	1.37	0.84	0.51	0.34	0.26	0.07
Corrected OD	2.37	1.84	1.3	0.77	0.44	0.27	0.19	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human HGFR spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Serum(n=8)	84-101	96
Plasma(n=8)	92-105	102
Cell Culture Supernates(n=8)	96-108	105

| Linearity

Samples were spiked with high concentrations of Human HGFR and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Serum(n=4)	Plasma(n=4)	Cell Culture Supernates(n=4)
1 : 2	Range (%)	84-95	88-96	90-110
1 : 2	Average (%)	91	93	96
1 : 4	Range (%)	89-103	88-108	105-115
1 : 4	Average (%)	94	98	108